She graduated as a journalist and film critic from the State University of Moscow. In the mid-90s she was one of the organizers of Cine Fantom, an experimental cinema club in Moscow. She has taken part in several exhibitions, biennales and festivals, including the Ars Electronica, Linz, 1997 and 1999; MOCA, Los Angeles 2000; Walker Art Center, Minneapolis 2000; the Montreal Biennale, Montreal 2000; VIPER Festival, Basel 2000; MediaZlounge, New York 2000; Totalmuseum, Seoul 2001; the Sao Paulo Biennale, 2002, La Casa Encendida, Madrid, 2003; the MEIAC, Badajoz 2006; Transmediale, Berlin, 2007. Lialina has worked on projects as director of net art and given seminars and conferences on the new media. She has written theoretical texts on the Internet on what she referred to as "digital folklore" and "Web vernacular". She is a professor at the Merz Akademie in Stuttgart, Germany.